Characterization and comparative analysis of promoters from three plant pararetroviruses associated with Dahlia (Dahlia variabilis).
Identifieur interne : 000361 ( Main/Exploration ); précédent : 000360; suivant : 000362Characterization and comparative analysis of promoters from three plant pararetroviruses associated with Dahlia (Dahlia variabilis).
Auteurs : C V Almeyda [États-Unis] ; G. Raikhy ; H R PappuSource :
- Virus genes [ 1572-994X ] ; 2015.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Caulimovirus (génétique), Caulimovirus (isolement et purification), Clonage moléculaire (MeSH), Dahlia (virologie), Fusion artificielle de gènes (MeSH), Glucuronidase (analyse), Glucuronidase (génétique), Gènes rapporteurs (MeSH), Régions promotrices (génétique) (MeSH), Rétrovirus endogènes (génétique), Rétrovirus endogènes (isolement et purification), Site d'initiation de la transcription (MeSH), Tabac (virologie), Vecteurs génétiques (MeSH), Électroporation (MeSH), Éléments de régulation transcriptionnelle (MeSH).
- MESH :
- analyse : Glucuronidase.
- génétique : Caulimovirus, Glucuronidase, Rétrovirus endogènes.
- isolement et purification : Caulimovirus, Rétrovirus endogènes.
- virologie : Dahlia, Tabac.
- Analyse de profil d'expression de gènes, Clonage moléculaire, Fusion artificielle de gènes, Gènes rapporteurs, Régions promotrices (génétique), Site d'initiation de la transcription, Vecteurs génétiques, Électroporation, Éléments de régulation transcriptionnelle.
English descriptors
- KwdEn :
- Artificial Gene Fusion (MeSH), Caulimovirus (genetics), Caulimovirus (isolation & purification), Cloning, Molecular (MeSH), Dahlia (virology), Electroporation (MeSH), Endogenous Retroviruses (genetics), Endogenous Retroviruses (isolation & purification), Gene Expression Profiling (MeSH), Genes, Reporter (MeSH), Genetic Vectors (MeSH), Glucuronidase (analysis), Glucuronidase (genetics), Promoter Regions, Genetic (MeSH), Regulatory Elements, Transcriptional (MeSH), Tobacco (virology), Transcription Initiation Site (MeSH).
- MESH :
- chemical , analysis : Glucuronidase.
- genetics : Caulimovirus, Endogenous Retroviruses, Glucuronidase.
- isolation & purification : Caulimovirus, Endogenous Retroviruses.
- virology : Dahlia, Tobacco.
- Artificial Gene Fusion, Cloning, Molecular, Electroporation, Gene Expression Profiling, Genes, Reporter, Genetic Vectors, Promoter Regions, Genetic, Regulatory Elements, Transcriptional, Transcription Initiation Site.
Abstract
Two distinct caulimoviruses, Dahlia mosaic virus (DMV) and Dahlia common mosaic virus (DCMV), and an endogenous plant pararetroviral sequence (DvEPRS, formerly known as DMV-D10) were reported from dahlia (Dahlia spp). Promoter elements from these dahlia-associated pararetroviruses were identified and characterized. The TATA box, the CAAT box, the transcription start site, the polyadenylation signal, and regulation factors, characteristic of caulimovirus promoters, were present in each of these promoter regions. Each of the promoter regions was separately cloned into a binary vector containing β-glucuronidase (GUS) reporter gene and delivered into Agrobacterium tumefaciens by electroporation followed by agroinfiltration into Nicotiana benthamiana. The activity of the 35S promoter homologs was determined by transient expression of the GUS gene both in qualitative and quantitative assays. The length of the promoter regions in DMV, DCMV, and DvEPRS corresponded to 438, 439, and 259 bp, respectively. Quantitative GUS assays showed that the promoters from DMV and DCMV resulted in higher levels of gene expression compared to that of DvEPRS in N. benthamiana leaf tissue. Significant differences were observed among the three promoters (p < 0.001). Qualitative GUS assays were consistent with quantitative GUS results. This study provides important information on new promoters for prospect applications as novel promoters for their potential use in foreign gene expression in plants.
DOI: 10.1007/s11262-015-1196-7
PubMed: 25947569
Affiliations:
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Raikhy</name></author><author><name sortKey="Pappu, H R" sort="Pappu, H R" uniqKey="Pappu H" first="H R" last="Pappu">H R Pappu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25947569</idno><idno type="pmid">25947569</idno><idno type="doi">10.1007/s11262-015-1196-7</idno><idno type="wicri:Area/Main/Corpus">000345</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000345</idno><idno type="wicri:Area/Main/Curation">000345</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000345</idno><idno type="wicri:Area/Main/Exploration">000345</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization and comparative analysis of promoters from three plant pararetroviruses associated with Dahlia (Dahlia variabilis).</title><author><name sortKey="Almeyda, C V" sort="Almeyda, C V" uniqKey="Almeyda C" first="C V" last="Almeyda">C V Almeyda</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Washington State University, Pullman, WA, 99163, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Washington State University, Pullman, WA, 99163</wicri:regionArea><wicri:noRegion>99163</wicri:noRegion></affiliation></author><author><name sortKey="Raikhy, G" sort="Raikhy, G" uniqKey="Raikhy G" first="G" last="Raikhy">G. Raikhy</name></author><author><name sortKey="Pappu, H R" sort="Pappu, H R" uniqKey="Pappu H" first="H R" last="Pappu">H R Pappu</name></author></analytic><series><title level="j">Virus genes</title><idno type="eISSN">1572-994X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Artificial Gene Fusion (MeSH)</term><term>Caulimovirus (genetics)</term><term>Caulimovirus (isolation & purification)</term><term>Cloning, Molecular (MeSH)</term><term>Dahlia (virology)</term><term>Electroporation (MeSH)</term><term>Endogenous Retroviruses (genetics)</term><term>Endogenous Retroviruses (isolation & purification)</term><term>Gene Expression Profiling (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Glucuronidase (analysis)</term><term>Glucuronidase (genetics)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Regulatory Elements, Transcriptional (MeSH)</term><term>Tobacco (virology)</term><term>Transcription Initiation Site (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Caulimovirus (génétique)</term><term>Caulimovirus (isolement et purification)</term><term>Clonage moléculaire (MeSH)</term><term>Dahlia (virologie)</term><term>Fusion artificielle de gènes (MeSH)</term><term>Glucuronidase (analyse)</term><term>Glucuronidase (génétique)</term><term>Gènes rapporteurs (MeSH)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Rétrovirus endogènes (génétique)</term><term>Rétrovirus endogènes (isolement et purification)</term><term>Site d'initiation de la transcription (MeSH)</term><term>Tabac (virologie)</term><term>Vecteurs génétiques (MeSH)</term><term>Électroporation (MeSH)</term><term>Éléments de régulation transcriptionnelle (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Caulimovirus</term><term>Endogenous Retroviruses</term><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Caulimovirus</term><term>Glucuronidase</term><term>Rétrovirus endogènes</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Caulimovirus</term><term>Endogenous Retroviruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Caulimovirus</term><term>Rétrovirus endogènes</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Dahlia</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Dahlia</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Artificial Gene Fusion</term><term>Cloning, Molecular</term><term>Electroporation</term><term>Gene Expression Profiling</term><term>Genes, Reporter</term><term>Genetic Vectors</term><term>Promoter Regions, Genetic</term><term>Regulatory Elements, Transcriptional</term><term>Transcription Initiation Site</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Clonage moléculaire</term><term>Fusion artificielle de gènes</term><term>Gènes rapporteurs</term><term>Régions promotrices (génétique)</term><term>Site d'initiation de la transcription</term><term>Vecteurs génétiques</term><term>Électroporation</term><term>Éléments de régulation transcriptionnelle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two distinct caulimoviruses, Dahlia mosaic virus (DMV) and Dahlia common mosaic virus (DCMV), and an endogenous plant pararetroviral sequence (DvEPRS, formerly known as DMV-D10) were reported from dahlia (Dahlia spp). Promoter elements from these dahlia-associated pararetroviruses were identified and characterized. The TATA box, the CAAT box, the transcription start site, the polyadenylation signal, and regulation factors, characteristic of caulimovirus promoters, were present in each of these promoter regions. Each of the promoter regions was separately cloned into a binary vector containing β-glucuronidase (GUS) reporter gene and delivered into Agrobacterium tumefaciens by electroporation followed by agroinfiltration into Nicotiana benthamiana. The activity of the 35S promoter homologs was determined by transient expression of the GUS gene both in qualitative and quantitative assays. The length of the promoter regions in DMV, DCMV, and DvEPRS corresponded to 438, 439, and 259 bp, respectively. Quantitative GUS assays showed that the promoters from DMV and DCMV resulted in higher levels of gene expression compared to that of DvEPRS in N. benthamiana leaf tissue. Significant differences were observed among the three promoters (p < 0.001). Qualitative GUS assays were consistent with quantitative GUS results. This study provides important information on new promoters for prospect applications as novel promoters for their potential use in foreign gene expression in plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25947569</PMID><DateCompleted><Year>2016</Year><Month>04</Month><Day>21</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1572-994X</ISSN><JournalIssue CitedMedium="Internet"><Volume>51</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Virus genes</Title><ISOAbbreviation>Virus Genes</ISOAbbreviation></Journal><ArticleTitle>Characterization and comparative analysis of promoters from three plant pararetroviruses associated with Dahlia (Dahlia variabilis).</ArticleTitle><Pagination><MedlinePgn>96-104</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11262-015-1196-7</ELocationID><Abstract><AbstractText>Two distinct caulimoviruses, Dahlia mosaic virus (DMV) and Dahlia common mosaic virus (DCMV), and an endogenous plant pararetroviral sequence (DvEPRS, formerly known as DMV-D10) were reported from dahlia (Dahlia spp). Promoter elements from these dahlia-associated pararetroviruses were identified and characterized. The TATA box, the CAAT box, the transcription start site, the polyadenylation signal, and regulation factors, characteristic of caulimovirus promoters, were present in each of these promoter regions. Each of the promoter regions was separately cloned into a binary vector containing β-glucuronidase (GUS) reporter gene and delivered into Agrobacterium tumefaciens by electroporation followed by agroinfiltration into Nicotiana benthamiana. The activity of the 35S promoter homologs was determined by transient expression of the GUS gene both in qualitative and quantitative assays. The length of the promoter regions in DMV, DCMV, and DvEPRS corresponded to 438, 439, and 259 bp, respectively. Quantitative GUS assays showed that the promoters from DMV and DCMV resulted in higher levels of gene expression compared to that of DvEPRS in N. benthamiana leaf tissue. Significant differences were observed among the three promoters (p < 0.001). Qualitative GUS assays were consistent with quantitative GUS results. This study provides important information on new promoters for prospect applications as novel promoters for their potential use in foreign gene expression in plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Almeyda</LastName><ForeName>C V</ForeName><Initials>CV</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Washington State University, Pullman, WA, 99163, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Raikhy</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Pappu</LastName><ForeName>H R</ForeName><Initials>HR</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>05</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Virus Genes</MedlineTA><NlmUniqueID>8803967</NlmUniqueID><ISSNLinking>0920-8569</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019677" MajorTopicYN="N">Artificial Gene Fusion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017796" MajorTopicYN="N">Caulimovirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031944" 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IdType="pubmed">2535536</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Pappu, H R" sort="Pappu, H R" uniqKey="Pappu H" first="H R" last="Pappu">H R Pappu</name><name sortKey="Raikhy, G" sort="Raikhy, G" uniqKey="Raikhy G" first="G" last="Raikhy">G. Raikhy</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Almeyda, C V" sort="Almeyda, C V" uniqKey="Almeyda C" first="C V" last="Almeyda">C V Almeyda</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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